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mouse brie crispr knockout pooled library plasmid library  (Lucigen Corp)

 
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    Lucigen Corp mouse brie crispr knockout pooled library plasmid library
    Mouse Brie Crispr Knockout Pooled Library Plasmid Library, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+brie+crispr+knockout+pooled+library+plasmid+library/pm41565761-83-6-24?v=Lucigen+Corp
    Average 86 stars, based on 1 article reviews
    mouse brie crispr knockout pooled library plasmid library - by Bioz Stars, 2026-08
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    mouse brie crispr knockout pooled library plasmid library  (Lucigen Corp)
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    The genome-wide screen reveals <t>DNA</t> damage-related pathways in the activation of MERVL . ( A ) Workflow for the genome-wide CRISPR/Cas9 screen. GeCKO, Genome-scale <t>CRISPR-Cas9</t> <t>Knockout;</t> LTR, long terminal repeat; MOI, multiplicity of infection; FACS, fluorescence-activated cell sorting. ( B ) FACS analysis showing the percentage of MERVL -activated cells after 5 days of GeCKO library treatment. ( C ) Ranking of previously reported MERVL -negative regulators (left) and positive regulators (right). Genes are ranked by RRA score. RRA, robust ranking aggregation. ( D ) Top five Gene Ontology terms with the lowest P -value identified through the GeCKO screen. ( E ) Immunofluorescence analysis in wild-type mESCs depicting MERVL-Gag (red), γH2AX (yellow), and DAPI (gray). Bar: 10 μm. ( F ) Quantification of γH2AX fluorescence intensity in MERVL + and MERVL − cells. **** P -value < 0.0001 by t -test. ( G ) Immunofluorescence analysis demonstrating MERVL-Gag (red) in mESCs treated with DMSO and 1 and 10 μM etoposide. DMSO, dimethylsulfoxide; ETO, etoposide. Bar: 10 μm. ( H ) qPCR analysis of MERVL expression in mESCs treated with DMSO and 1 and 10 μM etoposide. * P -value < 0.05, **** P -value < 0.0001 by one-way ANOVA, error bars represent the SD. ( I ) Western blot analysis of MERVL-Gag and γH2AX in mESCs collected 1 day after 7 Gy of X-ray irradiation or untreated controls.
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    The genome-wide screen reveals <t>DNA</t> damage-related pathways in the activation of MERVL . ( A ) Workflow for the genome-wide CRISPR/Cas9 screen. GeCKO, Genome-scale <t>CRISPR-Cas9</t> <t>Knockout;</t> LTR, long terminal repeat; MOI, multiplicity of infection; FACS, fluorescence-activated cell sorting. ( B ) FACS analysis showing the percentage of MERVL -activated cells after 5 days of GeCKO library treatment. ( C ) Ranking of previously reported MERVL -negative regulators (left) and positive regulators (right). Genes are ranked by RRA score. RRA, robust ranking aggregation. ( D ) Top five Gene Ontology terms with the lowest P -value identified through the GeCKO screen. ( E ) Immunofluorescence analysis in wild-type mESCs depicting MERVL-Gag (red), γH2AX (yellow), and DAPI (gray). Bar: 10 μm. ( F ) Quantification of γH2AX fluorescence intensity in MERVL + and MERVL − cells. **** P -value < 0.0001 by t -test. ( G ) Immunofluorescence analysis demonstrating MERVL-Gag (red) in mESCs treated with DMSO and 1 and 10 μM etoposide. DMSO, dimethylsulfoxide; ETO, etoposide. Bar: 10 μm. ( H ) qPCR analysis of MERVL expression in mESCs treated with DMSO and 1 and 10 μM etoposide. * P -value < 0.05, **** P -value < 0.0001 by one-way ANOVA, error bars represent the SD. ( I ) Western blot analysis of MERVL-Gag and γH2AX in mESCs collected 1 day after 7 Gy of X-ray irradiation or untreated controls.
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    The genome-wide screen reveals DNA damage-related pathways in the activation of MERVL . ( A ) Workflow for the genome-wide CRISPR/Cas9 screen. GeCKO, Genome-scale CRISPR-Cas9 Knockout; LTR, long terminal repeat; MOI, multiplicity of infection; FACS, fluorescence-activated cell sorting. ( B ) FACS analysis showing the percentage of MERVL -activated cells after 5 days of GeCKO library treatment. ( C ) Ranking of previously reported MERVL -negative regulators (left) and positive regulators (right). Genes are ranked by RRA score. RRA, robust ranking aggregation. ( D ) Top five Gene Ontology terms with the lowest P -value identified through the GeCKO screen. ( E ) Immunofluorescence analysis in wild-type mESCs depicting MERVL-Gag (red), γH2AX (yellow), and DAPI (gray). Bar: 10 μm. ( F ) Quantification of γH2AX fluorescence intensity in MERVL + and MERVL − cells. **** P -value < 0.0001 by t -test. ( G ) Immunofluorescence analysis demonstrating MERVL-Gag (red) in mESCs treated with DMSO and 1 and 10 μM etoposide. DMSO, dimethylsulfoxide; ETO, etoposide. Bar: 10 μm. ( H ) qPCR analysis of MERVL expression in mESCs treated with DMSO and 1 and 10 μM etoposide. * P -value < 0.05, **** P -value < 0.0001 by one-way ANOVA, error bars represent the SD. ( I ) Western blot analysis of MERVL-Gag and γH2AX in mESCs collected 1 day after 7 Gy of X-ray irradiation or untreated controls.

    Journal: Nucleic Acids Research

    Article Title: Ints7 deficiency activates DNA damage response to elicit resurgence of endogenous retrovirus MERVL and anastasis of embryonic stem cells

    doi: 10.1093/nar/gkaf797

    Figure Lengend Snippet: The genome-wide screen reveals DNA damage-related pathways in the activation of MERVL . ( A ) Workflow for the genome-wide CRISPR/Cas9 screen. GeCKO, Genome-scale CRISPR-Cas9 Knockout; LTR, long terminal repeat; MOI, multiplicity of infection; FACS, fluorescence-activated cell sorting. ( B ) FACS analysis showing the percentage of MERVL -activated cells after 5 days of GeCKO library treatment. ( C ) Ranking of previously reported MERVL -negative regulators (left) and positive regulators (right). Genes are ranked by RRA score. RRA, robust ranking aggregation. ( D ) Top five Gene Ontology terms with the lowest P -value identified through the GeCKO screen. ( E ) Immunofluorescence analysis in wild-type mESCs depicting MERVL-Gag (red), γH2AX (yellow), and DAPI (gray). Bar: 10 μm. ( F ) Quantification of γH2AX fluorescence intensity in MERVL + and MERVL − cells. **** P -value < 0.0001 by t -test. ( G ) Immunofluorescence analysis demonstrating MERVL-Gag (red) in mESCs treated with DMSO and 1 and 10 μM etoposide. DMSO, dimethylsulfoxide; ETO, etoposide. Bar: 10 μm. ( H ) qPCR analysis of MERVL expression in mESCs treated with DMSO and 1 and 10 μM etoposide. * P -value < 0.05, **** P -value < 0.0001 by one-way ANOVA, error bars represent the SD. ( I ) Western blot analysis of MERVL-Gag and γH2AX in mESCs collected 1 day after 7 Gy of X-ray irradiation or untreated controls.

    Article Snippet: The plasmid DNA library was amplified following the protocol provided by Addgene ( http://www.addgene.org/pooled-library/broadgpp-mouse-knockout-brie/ ).

    Techniques: Genome Wide, Activation Assay, CRISPR, Knock-Out, Infection, Fluorescence, FACS, Immunofluorescence, Expressing, Western Blot, Irradiation